Bacteriostatic enterochelin-specific immunoglobulin from normal human serum

Hizentra, INN-human normal immunoglobulin

PREPARATION OF ENRICHED IMMUNOGLOBULIN M AND IMMUNOGLOBULIN A FROM HUMAN PLASMA

As IgM and IgA-enriched preparations are needed to complete the immunotherapeutic spectrum, a simple procedure is described for the preparation of IgM and IgA enriched immunoglobulins. Fraction III which was prepared by cold ethanol fractionation was treated by octanoic acid followed by ethanol precipitation and ion-exchange chromatography using Sephadex DEAE A-50 and 0.1 M tris-D.35M NaCI ...

Structural analysis of the N-glycans from human immunoglobulin A1: comparison of normal human serum immunoglobulin A1 with that isolated from patients with rheumatoid arthritis.

The primary structures of the N-linked oligosaccharides from normal human serum IgA1 were determined by a combination of sequential exoglycosidase digestion, Bio-Gel P-4 chromatography, anion-exchange chromatography and one-dimensional n.m.r. spectroscopy. Three major N-linked disialylated biantennary-complex-type structures were found (55%). The remaining N-linked oligosaccharides consisted of...

Bacteriostatic activity of serum against staphylococci.

Cybulska, Janina (State Institute of Hygiene, Warsaw, Poland), and J. Jeljaszewicz. Bacteriostatic activity of serum against staphylococci. J. Bacteriol. 91:953-962. 1966.-Antistaphylococcal activity of normal serum against strains exhibiting various patterns of coagulase, clumping-factor, and staphylokinase production is not connected with the presence of these factors. Purified coagulase does...

Tube Determination of Serum Bacteriostatic Activity

SYNOPSIS The growth of 100 staphylococcal strains in normal rabbit serum was studied. Determinations were carried out by the tube method previously described and by a new plate method, and the results agreed in both cases. The strains of staphylococci used differed in the production of bound coagulase, clumping factor, and staphylokinase, and exhibited different phage patterns. Their multiplica...